primary cells human kidney proximal tubular epithelial cells  (ATCC)

Journal: Life

Article Title: VSIG4 Induces Epithelial-Mesenchymal Transition of Renal Tubular Cells under High-Glucose Conditions

doi: 10.3390/life10120354

Figure Lengend Snippet: Upregulation of V-set immunoglobulin-domain-containing 4 (VSIG4) induced by HG condition in human proximal tubular epithelial cells (HK-2 cells). ( A ) HK-2 cells were incubated with HG for 6 h, 24 h, and 72 h. VSIG4 expression was determined by RT-PCR. ( B ) HK-2 cells were incubated with HG for 24 h, 48 h, and 72 h, and then subjected to Western blot analysis using anti-VSIG4 antibody. HG, high glucose.

Article Snippet: HK-2 cells, human kidney proximal tubular epithelial cells, and MDCK cells, Madin Darby Canine Kidney cells, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Incubation, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Life

Article Title: VSIG4 Induces Epithelial-Mesenchymal Transition of Renal Tubular Cells under High-Glucose Conditions

doi: 10.3390/life10120354

Figure Lengend Snippet: VSIG4 upregulation and changes in EMT markers in HK-2 cells grown under HG conditions. ( A ) Representative western blotting on fibrosis markers induced by HG ( B ) Expression analysis of VSIG4 and fibronectin protein using western blotting analysis. Abbreviations: HG, high glucose; EMT, epithelial-mesenchymal transition; MMP-9, matrix metalloproteinase-9. Values are expressed as mean ± SE. * p < 0.05 vs. LG.

Article Snippet: HK-2 cells, human kidney proximal tubular epithelial cells, and MDCK cells, Madin Darby Canine Kidney cells, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Western Blot, Expressing

Journal: Life

Article Title: VSIG4 Induces Epithelial-Mesenchymal Transition of Renal Tubular Cells under High-Glucose Conditions

doi: 10.3390/life10120354

Figure Lengend Snippet: Effect of VSIG4 inhibition on EMT markers and cell motility. ( A ) HK-2 cells were transfected with siVSIG4 under HG condition for 72 h, and then cells were collected. The expression of VSIG4 and fibrosis marker was analyzed using Western blotting. ( B ) Expression of VSIG4 after siVSIG4 transfection. ( C ) Representative Madin Darby Canine Kidney (MDCK) cells were incubated under HG condition for 24 h, 48 h, and 72 h, and then subjected to Western blot analysis using an anti-VSIG4 antibody. ( D ) Cell motility assay after siVSIG4 transfection was performed and MDCK cells were photographed using a CELENA TM digital imaging system. HG, high glucose; LG, low glucose; EMT, epithelial-mesenchymal transition; TGF-β, transforming growth factor-β; MMP-9, matrix metalloproteinase-9. Values are expressed as mean ± SE. * p < 0.01 vs. LG.

Article Snippet: HK-2 cells, human kidney proximal tubular epithelial cells, and MDCK cells, Madin Darby Canine Kidney cells, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Inhibition, Transfection, Expressing, Marker, Western Blot, Incubation, Motility Assay, Imaging